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Cryo-EM structure of ex vivo fibrils associated with extreme AA amyloidosis prevalence in a cat shelter

ABSTRACT: We present the 3.3 Å cryo-EM structure of an AA amyloid extracted post-mortem from the diseased kidney of a DSH cat with renal failure. The structure reveals a cross-beta architecture assembled from two 76-residue long proto-filaments. Despite >70% sequence homology to mouse and human SAA, the cat SAA variant adopts a unique amyloid fold exhibiting type-2 polymorphism. Based on shared disease profiles and almost identical sequences, we propose a similar amyloid fold of deposits identified previously in captive cheetah.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Felis Catus (cat) (felis Silvestris Catus)

TISSUE(S): Kidney

SUBMITTER: Giulia Mazzini

LAB HEAD: Giovanni Palladini

PROVIDER: PXD035851 | Pride | 2022-11-18

REPOSITORIES: Pride

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