A method for targeting a specified segment of DNA to a bacterial microorganelle

ABSTRACT: Our work presents a protocol for the encapsulation and subsequent affinity isolation of the proteinaceous compartment carrying a DNA segment. To this end, we have repurposed the microcompartment of Citrobacter freundii for propaneadiol utilisation (Pdu) and constructed a LacI transcription repressor that allows the assembly of a synthetic organelle containing the LacI-DNA complex. High-throughput sequencing of extracted DNA from the affinity-isolated MCPs shows that our strategy results in leads to packaging of a DNA segment carrying multiple LacI binding sites, but not the flanking regions.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Matej Butala  

LAB HEAD: Matej Butala

PROVIDER: PXD035877 | Pride | 2022-08-11

REPOSITORIES: Pride