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Saccharomyces cerevisiae LC-MSMS

ABSTRACT: IP-MS detection of proteins pulled down by Glc7-FLAG by mass spectrometry

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Yinsheng Wu

LAB HEAD: Shanshan Li

PROVIDER: PXD036005 | Pride | 2023-10-24

REPOSITORIES: Pride

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Glc7/PP1 dephosphorylates histone H3T11 to regulate autophagy and telomere silencing in response to nutrient availability.

Zhang Xinyu X Yu Qi Q Wu Yinsheng Y Zhang Yuan Y He Yi Y Wang Rongsha R Yu Xilan X Li Shanshan S

Cell discovery 20230711 1

How cells adapt their gene expression to nutritional changes remains poorly understood. Histone H3T11 is phosphorylated by pyruvate kinase to repress gene transcription. Here, we identify the protein phosphatase 1 (PP1), Glc7 as the enzyme that specifically dephosphorylates H3T11. We also characterize two novel Glc7-containing complexes and reveal their roles in regulating gene expression upon glucose starvation. Specifically, the Glc7-Sen1 complex dephosphorylates H3T11 to activate the transcri ...[more]

PMID: 37433812

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