Proteomics

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Proteomic analysis identifies molecular players and biological processes specific to SARS-CoV-2 exposure in endothelial cells

ABSTRACT: In this study we investigated the global proteomic response of human umbilical vein endothelial cells (HUVECs) after exposure to SARS-CoV-2. For this porpouse HUVECs were incubated with SARS-CoV-2 at a multiplicity of infection (MOI) of 1 and after different hours post-infection (hpi) samples were submitted to the 50% tissue-culture infective dose assay (TCID50), proteomic analysis and RT-qPCR measurements.To perform the relative quantitative label-free proteomic analysis, cells exposed to SARS-CoV-2 or MOCK were submitted to protein extraction, trypsin digestion and the tryptic peptides were analyzed in a Q-Exactive mass spectrometer. Data analysis of the differently abundant proteins of each cell model revealed distinct cell responses.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Endothelial Cell Of Umbilical Vein, Cell Culture

DISEASE(S): Severe Acute Respiratory Syndrome

SUBMITTER: Dilza Trevisan Silva  

LAB HEAD: Ana Marisa Chudzinski-Tavassi

PROVIDER: PXD036068 | Pride | 2022-10-15

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
MQ_proteinGroups_120h.txt Txt
MQ_proteinGroups_48h.txt Txt
QE_02831_20210218_RQ026_2021_HUVEC_MOCK48h_precolumn_001.raw Raw
QE_02832_20210219_RQ026_2021_HUVEC_MOCK48h_precolumn_002.raw Raw
QE_02833_20210219_RQ026_2021_HUVEC_MOCK48hN2_precolumn_001.raw Raw
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Publications

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for the severe pandemic of acute respiratory disease, coronavirus disease 2019 (COVID-19), experienced in the 21st century. The clinical manifestations range from mild symptoms to abnormal blood coagulation and severe respiratory failure. In severe cases, COVID-19 manifests as a thromboinflammatory disease. Damage to the vascular compartment caused by SARS-CoV-2 has been linked to thrombosis, triggered by an enhanc  ...[more]

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