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Proximity labeling facilitates defining neighborhood proteome of photosystem II oxygen evolution complex in a model cyanobacterium

ABSTRACT: APEX2-based proximity labeling coupled with mass spectrometry have great potential for spatiotemporal identification of proteins proximal to a protein complex of interest. Using this approach it is feasible to define the proteome neighborhood of important protein complexes in a popular photosynthetic model cyanobacterium Synechocystis sp. PCC6803 (hereafter named as Synechocystis). To this end, we developed a robust workflow for APEX2-based proximity labeling in Synechocystis, and used the workflow to identify proteins proximal to the photosystem II (PS II) oxygen evolution complex (OEC) through fusion APEX2 with a luminal OEC subunit, PsbO.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Synechocystis Sp. Pcc 6803

TISSUE(S): Cell Culture

SUBMITTER: Chengcheng Huang

LAB HEAD: Yingchun Wang

PROVIDER: PXD036113 | Pride | 2023-02-17

REPOSITORIES: Pride

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Proximity Labeling Facilitates Defining the Proteome Neighborhood of Photosystem II Oxygen Evolution Complex in a Model Cyanobacterium.

Xiao Zhen Z Huang Chengcheng C Ge Haitao H Wang Yan Y Duan Xiaoxiao X Wang Gaojie G Zheng Limin L Dong Jinghui J Huang Xiahe X Zhang Yuanya Y An Hongyu H Xu Wu W Wang Yingchun Y

Molecular & cellular proteomics : MCP 20221108 12

Ascorbate peroxidase (APEX)-based proximity labeling coupled with mass spectrometry has a great potential for spatiotemporal identification of proteins proximal to a protein complex of interest. Using this approach is feasible to define the proteome neighborhood of important protein complexes in a popular photosynthetic model cyanobacterium Synechocystis sp. PCC6803 (hereafter named as Synechocystis). To this end, we developed a robust workflow for APEX2-based proximity labeling in Synechocystis ...[more]

PMID: 36356940

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