Identification of phosphorylation sites of HA-ORP8 immunoprecipitated from cells
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: 
maomao pu
LAB HEAD: Wei Liu
PROVIDER: PXD036204 | Pride | 2023-10-24
REPOSITORIES: Pride
Publications
ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover.
Pu Maomao M Zheng Wenhui W Zhang Hongtao H Wan Wei W Peng Chao C Chen Xuebo X Liu Xinchang X Xu Zizhen Z Zhou Tianhua T Sun Qiming Q Neculai Dante D Liu Wei W
Protein & cell 20230901 9
Lipophagy, the selective engulfment of lipid droplets (LDs) by autophagosomes for lysosomal degradation, is critical to lipid and energy homeostasis. Here we show that the lipid transfer protein ORP8 is located on LDs and mediates the encapsulation of LDs by autophagosomal membranes. This function of ORP8 is independent of its lipid transporter activity and is achieved through direct interaction with phagophore-anchored LC3/GABARAPs. Upon lipophagy induction, ORP8 has increased localization on L ...[more]
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