Dataset Information

Comparative protein profiles of differentially temperature tolerant maize genotypes

ABSTRACT: Two maize genotypes exhibiting differential tolerance towards LT including the temperate grown Gurez local and its tropical counterpart GM6 were dissected for analysing and characterizing the functions of differentially regulated proteins (DRPs) in response to LT stress.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Salika Ramazan Naik

LAB HEAD: Riffat John

PROVIDER: PXD036246 | Pride | 2023-05-10

REPOSITORIES: Pride

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Publications

Comparative protein analysis of two maize genotypes with contrasting tolerance to low temperature.

Ramazan Salika S Jan Nelofer N John Riffat R

BMC plant biology 20230405 1

<h4>Background</h4>Low temperature (LT) stress is one of the major environmental stress factors affecting the growth and yield of maize (Zea mays L.). Hence, it is important to unravel the molecular mechanisms behind LT stress tolerance to improve molecular breeding in LT tolerant genotypes. In the present study, two maize genotypes viz. Gurez local from Kashmir Himalaya and tropical grown GM6, were dissected for their LT stress response in terms of accumulation of differentially regulated prote ...[more]

PMID: 37020183

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Project description:Several reports have described the involvement of miRNAs in abiotic stresses. However, their role in biotic stress or to beneficial microbes has not been fully explored. In order to understand on the epigenetic regulation in plant in response to nitrogen-fixing bacteria association, we analyzed the sRNA regulation in maize hybrids (Zea mays M-bM-^@M-^S UENF 506-8) inoculated with the beneficial diazotrophic bacteria (Herbaspirillum seropedicae). Deep sequencing analysis was carried out to identify the sRNAs regulated in maize during association with diazotrophic bacteria. For this analysis, maize plants were germinated in wet paper and put in hydroponic system with HoaglandM-bM-^@M-^Ys solution and then inoculated with H. seropedicae for seven days. Mock and inoculated plants were collected and total RNA from a pool of samples was extracted with Trizol reagent. The two sRNA libraries were sequenced by Illumina. The sequences were filtered to remove adaptors and contaminants rRNA and tRNAs, and sequences with 18-28 nt in length were selected. To identify the miRNAs present in these libraries, we used two strategies using the same website (http://srna-tools.cmp.uea.ac.uk): one to identify novel miRNAs using the maize genome (verson 2) and miRCat pipeline; and other to identify conserved miRNAs using the miRBase database (release 13.0, http://microrna.sanger.ac.uk) and miRProf pipeline. We identified 17 novel putative miRNAs candidates and mapped the precursor of these miRNAs in the maize genome. Furthermore, we identified 25 conserved miRNAs families and the differential expressions were analyzed with miRProf pipeline. The bioinformatics analysis of four up-regulated miRNAs (miR397, miR398, miR408 and miR528) in inoculated plant was validated using stemM-bM-^@M-^Sloop RT-PCR assay. Our findings contribute to increase the knowledge of the molecular relation between plants and endophytic bacteria. Screenning of sRNA transcriptome of maize plants inoculated with Herbaspirillum seropedicae after seven days

Dataset Information

Comparative protein profiles of differentially temperature tolerant maize genotypes

Publications

Comparative protein analysis of two maize genotypes with contrasting tolerance to low temperature.

Similar Datasets

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