Dataset Information

Expression of glycan receptors for influenza A viruses in mallards, chickens, and tufted ducks

ABSTRACT: Influenza A virus (IAV) pandemics result from interspecies transmission events within the avian reservoir and further into mammals including humans. Investigating the molecular basis for virus–host interactions enabling this process is vital to understand zoonotic IAV spread. Receptor incompatibility has been suggested to limit zoonotic IAV transmission from the wild bird reservoir as well as between different bird species. Using glycoproteomics, we have studied the repertoires of expressed glycan structures with focus on putative receptors for IAV in mallards, chickens and tufted ducks; three bird species with different roles in the zoonotic ecology of IAV. The methodology used could not only pinpoint specific glycan structures to the specific glycosylation sites of identified glycoproteins but could also be used to successfully discriminate α2,3- from α2,6-linked terminal sialic acids by careful analysis of oxonium ions released from glycopeptides during MS/MS (MS2), and MS/MS/MS (MS3). Our analysis clearly demonstrated that all three bird species can produce complex α2,3 and α2,6-linked Neu5Ac N-glycans including α2,3-linked sialyl Lewis structures, as well as both N- and O- glycans terminated with both α2,3 and α2,6-linked Neu5Ac. Furthermore, we reveal many similarities in the repertoires of expressed receptors both between the bird species investigated and to previously published data from pigs and humans. Our findings of sialylated glycan structures previously anticipated to be mammalian specific in all three bird species have major implications for our understanding of the role of receptor incompatibility in interspecies transmission of IAV.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Lung, Colon

DISEASE(S): Influenza

SUBMITTER: Ekaterina Mirgorodskaya

LAB HEAD: Patrik Ellström

PROVIDER: PXD036263 | Pride | 2024-04-18

REPOSITORIES: Pride

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Publications

Expression of influenza A virus glycan receptor candidates in mallard, chicken, and tufted duck.

Nilsson Jonas J Eriksson Per P Naguib Mahmoud M MM Jax Elinor E Sihlbom Carina C Olsson Britt-Marie BM Lundkvist Åke Å Olsen Björn B Järhult Josef D JD Larson Göran G Ellström Patrik P

Glycobiology 20240401 3

Influenza A virus (IAV) pandemics result from interspecies transmission events within the avian reservoir and further into mammals including humans. Receptor incompatibility due to differently expressed glycan structures between species has been suggested to limit zoonotic IAV transmission from the wild bird reservoir as well as between different bird species. Using glycoproteomics, we have studied the repertoires of expressed glycan structures with focus on putative sialic acid-containing glyca ...[more]

PMID: 38127648

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Dataset Information

Expression of glycan receptors for influenza A viruses in mallards, chickens, and tufted ducks

Publications

Expression of influenza A virus glycan receptor candidates in mallard, chicken, and tufted duck.

Similar Datasets

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