D133 ORF10 immunoprecipitation from WT and TorsinKO cells

ABSTRACT: WT and TorsinKO HeLa cells were transfected with d133-ORF10-FLAG from KSHV. The protein was immunoprecipitated with anti-FLAG beads to investigate proteins contained within nuclear envelope blebs. A control sample with no specific immunoprecipitation was compared to the co-immunoprecipitations. Eluting proteins were ran into an SDS-PAGE gel and extracted for mass spectrometry analysis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Sarah Prophet  

LAB HEAD: Christian Schlieker

PROVIDER: PXD036264 | Pride | 2022-08-25

REPOSITORIES: Pride