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T4 protein MotB expression in Escherichia coli, HPLC-MS

ABSTRACT: We investigated the effect of the T4 MotB protein on E. coli gene expression. E. coli BL21 (DE3) containing either pNW129 or pNW129-MotB were grown to early log phase (OD600 ~ 0.3) then induced with 0.2% arabinose for 20 minutes. T4 phage added to the culture at MOI10. Cells were then harvested at 5 min.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Bokyung Son

LAB HEAD: Deborah M. Hinton

PROVIDER: PXD036347 | Pride | 2022-10-13

REPOSITORIES: Pride

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Setting Up a Better Infection: Overexpression of the Early Bacteriophage T4 Gene <i>motB</i> During Infection Results in a More Favorable tRNA Pool for the Phage.

Son Bokyung B Patterson-West Jennifer J Thompson Christine O CO Iben James R JR Hinton Deborah M DM

PHAGE (New Rochelle, N.Y.) 20220919 3

<h4>Background</h4><i>:</i> Although many bacteriophage T4 early genes are nonessential with unknown functions, they are believed to aid in the takeover of the <i>Escherichia coli</i> host. Understanding the functions of these genes could be helpful to develop novel antibacterial strategies. MotB, encoded by a previously uncharacterized T4 early gene, is a DNA-binding protein that compacts the host nucleoid and alters host gene expression.<h4>Methods</h4><i>:</i> MotB structure was predicted by ...[more]

PMID: 36196375

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