Proteomics

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Proteomic profiling of glioblastoma stem cells and their small extracellular vesicles

ABSTRACT: The heterogeneous nature of glioblastoma stem-like cells (GSCs) creates hurdles in developing effective therapies against glioblastoma, making it extremely difficult to eradicate. In this part of the project, we investigated the potential role of GSCs-derived small extracellular vesicles (sEVs) in glioblastoma heterogeneity, plasticity, and aggressiveness with the particular focus on their complexity in term of proteins. Proteome profiling of sEVs and their respective cell lines showed that GSCs-derived sEVs retain their subtype characteristics and are enriched in proteins playing a role in amino acids, carboxylic acids, organic acids transmembrane transport, and growth factor binding. In summary, we revealed the protein content of GSCs-derived sEVs and unveiled their potential contribution to tumor heterogeneity and critical cellular processes commonly deregulated in glioblastoma.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Glioblastoma Multiforme Cancer Stem Cell

DISEASE(S): Glioblastoma

SUBMITTER: Tolga Lokumcu  

LAB HEAD: Dr. Violaine Goidts

PROVIDER: PXD036359 | Pride | 2024-05-21

REPOSITORIES: Pride

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Publications

Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.

Lokumcu Tolga T   Iskar Murat M   Schneider Martin M   Helm Dominic D   Klinke Glynis G   Schlicker Lisa L   Bethke Frederic F   Müller Gabriele G   Richter Karsten K   Poschet Gernot G   Phillips Emma E   Goidts Violaine V  

ACS nano 20240111 3

Glioblastoma is a deadly brain tumor for which there is no cure. The presence of glioblastoma stem-like cells (GSCs) contributes to the heterogeneous nature of the disease and makes developing effective therapies challenging. Glioblastoma cells have been shown to influence their environment by releasing biological nanostructures known as extracellular vesicles (EVs). Here, we investigated the role of GSC-derived nanosized EVs (<200 nm) in glioblastoma heterogeneity, plasticity, and aggressivenes  ...[more]

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