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Proteomic analysis of cuticle protein in Nilaparvata lugens

ABSTRACT: The brown planthopper (BPH, Nilaparvata lugens) is the most destructive pest of rice and causes serious economic damage in Asia. Understanding the composition of Nilaparvata lugens cuticle protein will help pest control. In this study, Nilaparvata lugens cuticle was disserted, and underwent shotgun MS/MS analysis.

INSTRUMENT(S):

ORGANISM(S): Nilaparvata Lugens

TISSUE(S): Skin

SUBMITTER: Haijian Huang

LAB HEAD: Hai-Jian Huang

PROVIDER: PXD036431 | Pride | 2022-12-12

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	LW_20161114_Pan_Penglu.raw	Raw
	Nl_translate_eve.fasta	Fasta
	identifiedPeptides	Other
	identifiedProteinGroups	Other

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proteome analysis of Nilaparvata lugens cuticle

Project description:The brown planthopper (BPH, Nilaparvata lugens) is the most destructive pest of rice and causes serious economic damage in Asia. Understanding the composition of Nilaparvata lugens protein will help pest control. In this study, shotgun MS/MS analysis was performed.

2023-10-24 | PXD044065 | Pride

proteome analysis of in Nilaparvata lugens

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The Daphnia cuticle proteome - partial cuticle samples

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ACL1-HD-Zip IV complex revealed common mechanism in rice response to brown planthopper and drought. Being a small peptide, ACL1 might function through forming complex with other proteins. So that we searched the possible interacting proteins of ACL1 using the TurboID system.

Project description:Brown planthopper (BPH) is the most notorious insect pest to rice. Drought is the most commonly occurring global adversity. BPH infestation caused adaxially-rolled leaves and shrunk bulliform cells similar to drought. The bulliform-cell characteristic gene, ACL1, negatively regulated BPH resistance and drought tolerance, with decreased cuticular wax in ACL1-D, which resulted in quicker water losing. ACL1 was specifically expressed in epidermis. TurboID system and various biochemical assays revealed that ACL1 interacted with the epidermal-characteristic HD-Zip IV ROCs. ROC4 and ROC5 positively regulated BPH resistance and drought tolerance through modulating cuticular wax and bulliform cells respectively. Overexpression of ROC4 and ROC5 both rescued ACL1-D in various related phenotypes simultaneously. Moreover, ACL1 competed with ROC4 and ROC5 in homo-dimerization and hetero-dimerization. Altogether, we illustrated that ACL1-ROCs complex synergistically mediate drought tolerance and BPH resistance through regulating cuticular wax and bulliform cells in rice, a new mechanism which might facilitate BPH resistance breeding.

2024-08-13 | PXD052228 | Pride

Identification and analysis of brown planthopper-responsive microRNAs in the resistant and susceptible rice plants

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Comprehensive Analysis of leaf cuticle proteome from B. juncea, B. nigra, B. rapa, and R. sativus leaf shed lights on diverse functions of differentially abundant classical and non-classical secretory targets Comprehensive Analysis of leaf cuticle proteome from B. juncea, B. nigra, B. rapa, and R. sativus leaf shed lights on diverse functions of differentially abundant classical and non-classical secretory targets

Project description:The primary sensor for environmental cues, the cuticle is an essential plant agronomical trait in crop plants. The extracellular cuticle proteome in crop plants is little understood. We performed a quantitative shotgun proteomics analysis on four economically relevant Brassicaceae members: B. juncea, B. nigra, B. rapa, and R. sativus. SignalP and SecretomeP predicted 323 secretory cuticle proteins, of which, 183 proteins were classically secreted and 140 proteins were non-classically secreted. QuickGO analysis revealed that most of these proteins had metabolic activity related to proteins (14%) and lipids (14%) as well as catalytic activity (19%) and defence response roles (18%). Some of the most abundant secreted proteins linked with lipid metabolism (GDSL esterase lipase, ESM1), catalytic activity (glucanase, myrosinase), and oxidoreductases (peroxidase, ascorbate peroxidase) were validated by biochemical investigation and western blotting. Also, the abundant non-classical secreted proteins including malate dehydrogenase, bisphosphatase, catalase, and fructose 1-6 showed in vitro activities. According to the results of the current proteomics study, the active enzymes in the cuticle may serve a variety of functions at the first line of defence. Cutinsomes (esterified nanostructures) which are thought to be involved in cuticle assembly through non enzymatic mechanism were synthesized invitro and validated by FTIR and UV visible spectroscopy. We proposed that cuticle proteins and cutinsomes co-ordinately might be involved in cuticle depositions on the Brassica leaves. In future, cutinsomes may thus also be studied for exploring their applications such as Bioplastics from underutilized Bioresources in India like Brassica agrowastes to reduce environmental pollution.

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