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Global analysis of lysine 2-hydroxyisobutyrylation in Glycine max

ABSTRACT: Lysine 2-hydroxyisobutyrylation (Khib) is a novel naturally occurring PTM, which is firstly discovered in histone. The system Khib detection at proteomics level has been performed in various species and tissues for the function and role characterization of Khib in biological activities. However, the Khib study in plant species is relatively less and the plant leaf, one of the critical plant organ, haven’t been studied. In the present study, the first root tissues lysine 2-hydroxyisobutyrylome analysis was performed in soybean specie with antibody immunoprecipitation affinity and high resolution mass spectrometry-based proteomics. Bioinformatics analyses including function classification, subcellular location predication, gene ontology enrichment and pathway enrichment were conducted to demonstrate the roles of these Khib sites and proteins in soybeanleaf.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Leaf

SUBMITTER: Xianliang Ma

LAB HEAD: Geng Li

PROVIDER: PXD036505 | Pride | 2022-09-20

REPOSITORIES: Pride

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