Proteomics

Dataset Information

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Drosophila S2s Elongator identification


ABSTRACT: A collection of stable Drosophila S2 cell lines was established, each one expressing a (His)-PC SNAP tagged version of each of the six Elongator subunits. From these stable lines, the endogenous Elongator complex can be recovered by pulling on the exogenous subunit via the PC tag. This allowed us to test which subunit can be over-expressed and used to recover the full Elongator complex for further in vitro experiments.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: Vicente José Planelles-Herrero  

LAB HEAD: Emmanuel derivery

PROVIDER: PXD036556 | Pride | 2022-09-15

REPOSITORIES: Pride

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