Proteomics

Dataset Information

Aurora A phosphorylates PKM2 at T45.

ABSTRACT: Flag-PKM2 was expressed in H1299 cells. Then the H1299 cells were treated with DMSO or 100nM MLN8237. Then, cell lysate was incubated with anti-Flag M2-agarose. Elutes were separated with 10% SDS-PAGE. The gel was stained with Coomassie Brilliant Blue. Visualized bands were excised and subjected to LC-MS/MS analysis. Then the phosphorylation sites of PKM2 were identified.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Aoxing Cheng  

LAB HEAD: Zhenye yang

PROVIDER: PXD036575 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
PKM-AuroraA-KD.xlsx Xlsx
PKM-AuroraA-WT.xlsx Xlsx
PKM2-AuroraA-KD.raw Raw
PKM2-AuroraA-WT.raw Raw
PKM2-DMSO.raw Raw
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