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Identification of biotinylated proteins through pull-down: ID and label-free quantification (Identification of TTP-interactome in RAW264.7 cells by TurboID proximity labelling).

ABSTRACT: Identification of biotinylated proteins through pull-down. RAW264.7 cells stably expressing a Dox-inducible TurboID-TTP fusion protein were treated with LPS or Epoxomicin for 4 h, followed by 15 min of biotin labeling in the cell medium. TurboID without fusion was used as a control.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: WeiQiang Chen

LAB HEAD: Gijs Versteeg

PROVIDER: PXD036714 | Pride | 2023-03-24

REPOSITORIES: Pride

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