Ontology highlight
ABSTRACT:
INSTRUMENT(S): timsTOF fleX
ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)
TISSUE(S): Cerebrospinal Fluid
SUBMITTER: Tuula Nyman
LAB HEAD: Tuula Nyman
PROVIDER: PXD036748 | Pride | 2023-07-20
REPOSITORIES: Pride
Scientific reports 20230612 1
The proteomic profile of extracellular vesicles (EVs) from cerebrospinal fluid (CSF) can reveal novel biomarkers for diseases of the brain. Here, we validate an ultrafiltration combined with size-exclusion chromatography (UF-SEC) method for isolation of EVs from canine CSF and probe the effect of starting volume on the EV proteomics profile. First, we performed a literature review of CSF EV articles to define the current state of art, discovering a need for basic characterisation of CSF EVs. Sec ...[more]