Control of intracellular pH and bicarbonate by CO2 diffusion into human sperm
Ontology highlight
ABSTRACT:
INSTRUMENT(S):
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Spermatozoon, Sperm
SUBMITTER: 
Svenja Victoria Kaufmann
LAB HEAD: Henning Urlaub
PROVIDER: PXD036819 | Pride | 2023-09-12
REPOSITORIES: Pride
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Publications
Control of intracellular pH and bicarbonate by CO<sub>2</sub> diffusion into human sperm.
Grahn Elena E Kaufmann Svenja V SV Askarova Malika M Ninov Momchil M Welp Luisa M LM Berger Thomas K TK Urlaub Henning H Kaupp U Benjamin UB
Nature communications 20230905 1
The reaction of CO<sub>2</sub> with H<sub>2</sub>O to form bicarbonate (HCO<sub>3</sub><sup>-</sup>) and H<sup>+</sup> controls sperm motility and fertilization via HCO<sub>3</sub><sup>-</sup>-stimulated cAMP synthesis. A complex network of signaling proteins participates in this reaction. Here, we identify key players that regulate intracellular pH (pH<sub>i</sub>) and HCO<sub>3</sub><sup>-</sup> in human sperm by quantitative mass spectrometry (MS) and kinetic patch-clamp fluorometry. The rest ...[more]
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