Proteomics

Dataset Information

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The accessory protein ORF6 contributes to SARS-CoV-2 pathogenesis by regulating host and viral gene expression

ABSTRACT: We generated a SARS-CoV-2 virus in the Wuhan background with a deletion of the Orf6 gene. We then performed mass spectrometry abundance proteomics and phosphoproteomics analysis of infected cells.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive

ORGANISM(S): Homo Sapiens (human) Severe Acute Respiratory Syndrome Coronavirus 2

TISSUE(S): Lung, Fibroblast

DISEASE(S): Covid-19

SUBMITTER: Mehdi Bouhaddou  

LAB HEAD: Nevan J. Krogan

PROVIDER: PXD036821 | Pride | 2024-02-16

REPOSITORIES: Pride

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Publications

Impact of SARS-CoV-2 ORF6 and its variant polymorphisms on host responses and viral pathogenesis.

Kehrer Thomas T   Cupic Anastasija A   Ye Chengjin C   Yildiz Soner S   Bouhaddou Mehdi M   Crossland Nicholas A NA   Barrall Erika A EA   Cohen Phillip P   Tseng Anna A   Çağatay Tolga T   Rathnasinghe Raveen R   Flores Daniel D   Jangra Sonia S   Alam Fahmida F   Mena Ignacio I   Aslam Sadaf S   Saqi Anjali A   Rutkowska Magdalena M   Ummadi Manisha R MR   Pisanelli Giuseppe G   Richardson R Blake RB   Veit Ethan C EC   Fabius Jacqueline M JM   Soucheray Margaret M   Polacco Benjamin J BJ   Ak Baran B   Marin Arturo A   Evans Matthew J MJ   Swaney Danielle L DL   Gonzalez-Reiche Ana S AS   Sordillo Emilia M EM   van Bakel Harm H   Simon Viviana V   Zuliani-Alvarez Lorena L   Fontoura Beatriz M A BMA   Rosenberg Brad R BR   Krogan Nevan J NJ   Martinez-Sobrido Luis L   García-Sastre Adolfo A   Miorin Lisa L  

Cell host & microbe 20230921 10

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) encodes several proteins that inhibit host interferon responses. Among these, ORF6 antagonizes interferon signaling by disrupting nucleocytoplasmic trafficking through interactions with the nuclear pore complex components Nup98-Rae1. However, the roles and contributions of ORF6 during physiological infection remain unexplored. We assessed the role of ORF6 during infection using recombinant viruses carrying a deletion or loss-of-functio  ...[more]

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