Semi-automated enrichment of AHA-containing newly-synthesized proteins with magnetic alkyne agarose beads

ABSTRACT: Development of an improved workflow for newly synthesized proteome analysis, based on combined pulsed metabolic labelling with L-azidohomoalanine (AHA) and semi-automated click chemistry-based enrichments with magnetic alkyne agarose beads.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Toman Bortecen  

LAB HEAD: Prof. Jeroen Krijgsveld

PROVIDER: PXD036886 | Pride | 2023-10-19

REPOSITORIES: Pride