Identification of substrates of ZDHHC20 in a mouse pancreatic carcinoma cell line

ABSTRACT: An shRNA screen to search for the mediators of pancreatic cancer metastasis identified palmitoyl transferase ZDHHC20 as a protein that mediates this process by promoting the outgrowth of metastatic cells in vivo. We sought to identify the substrates of ZDHHC20 by using an engineered mutant of ZDHHC20 with a Y181G substitution, which can selectively accommodate a synthetic lipid probe and transfer it to a protein substrate.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Pancreatic Cancer

SUBMITTER: Goran Tomic  

LAB HEAD: Julian Downward

PROVIDER: PXD036914 | Pride | 2023-02-09

REPOSITORIES: Pride