Identification of mono-ADPr readers by multilevel chromatin proteomics

ABSTRACT: We performed multilevel proteomics to identify mono-ADPr readers. Pulldowns using ADPribosylated peptides and nucleosomes, analysis of the chromatin proteome using targeted modulations of the ADPr system (H2O2 treatment, ARH3 KO, HPF1 KO). We reveal multiple mono-ADPr readers, including RNF114, a ubiquitin ligase recruited to DNA lesions through a zinc finger domain.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Thomas Colby  

LAB HEAD: Ivan Matic

PROVIDER: PXD037026 | Pride | 2023-04-28

REPOSITORIES: Pride