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Identification and analysis of the interaction network of African Swine Fever Virus D1133L with host proteins

ABSTRACT: African swine fever virus (ASFV) is a large double-stranded DNA virus encoding >150 open reading frames. Among them, ASFV-encoded D1133L was predicted to be a helicase but its specific function remains unknown. Since virus-host protein interactions are key to understanding viral protein function, we used co-immunoprecipitation combined with liquid chromatography-mass spectrometry to investigate D1133L. This study describes the interaction network of ASFV D1133L protein in porcine kidney PK-15 cells. Overall, 1471 host proteins are known to interact with D1133L.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Epithelial Cell

DISEASE(S): Disease Free

SUBMITTER: Hao Yu

LAB HEAD: Zheng Haixue

PROVIDER: PXD037029 | Pride | 2023-03-11

REPOSITORIES: Pride

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Identification and analysis of the interaction network of African swine fever virus D1133L with host proteins.

Hao Yu Y Yang Jinke J Yang Bo B Zhang Ting T Shi Xijuan X Yang Xing X Zhang Dajun D Zhao Dengshuai D Yan Wenqian W Chen Lingling L Liu Xiangtao X Zheng Haixue H Zhang Keshan K

Frontiers in microbiology 20221103

African swine fever (ASF) is a contagious and lethal hemorrhagic disease in pigs; its spread results in huge economic losses to the global pig industry. ASF virus (ASFV) is a large double-stranded DNA virus encoding >150 open reading frames. Among them, ASFV-encoded D1133L was predicted to be a helicase but its specific function remains unknown. Since virus-host protein interactions are key to understanding viral protein function, we used co-immunoprecipitation combined with liquid chromatograph ...[more]

PMID: 36406406

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