Proteomics

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Protein interaction partners of FRUITFULL in inflorescence meristems and pistils by IP-MS.

ABSTRACT: To study the possibility that the tissue-specific gene targets and regulation by FUL are due to a different composition in the FUL transcription factor (TF) protein complex/es in both tissues, we determined the protein-protein interactions of FUL in planta. Inflorescence meristems (IMs) of 35S:AP1-GR ap1 cal pFUL:FUL-GFP plants were collected to identify meristem-specific protein complexes of FUL, and stage 12 - 16 pistils of pFUL:FUL-GFP ful-1 plants were collected for the pistil-specific FUL protein complexes. Protein complexes were isolated by immunoprecipitation (IP) using anti-GFP antibodies and protein identification was performed using LC-MS/MS, followed by label-free protein quantification.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Flower, Inflorescence Meristem

SUBMITTER: Sjef Boeren  

LAB HEAD: Sjef Boeren

PROVIDER: PXD037091 | Pride | 2023-03-30

REPOSITORIES: Pride

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Publications

Dual specificity and target gene selection by the MADS-domain protein FRUITFULL.

van Mourik Hilda H   Chen Peilin P   Smaczniak Cezary C   Boeren Sjef S   Kaufmann Kerstin K   Bemer Marian M   Angenent Gerco C GC   Muino Jose M JM  

Nature plants 20230216 3

How transcription factors attain their target gene specificity and how this specificity may be modulated, acquiring different regulatory functions through the development of plant tissues, is an open question. Here we characterized different regulatory roles of the MADS-domain transcription factor FRUITFULL (FUL) in flower development and mechanisms modulating its activity. We found that the dual role of FUL in regulating floral transition and pistil development is associated with its different  ...[more]

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