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A gephyrin-like protein links cell division and polar elongation in Corynebacteriales

ABSTRACT: To discover missing players in corynebacterial cell division we used mass-spectrometry based interactomics using formaldehyde as crosslinker, starting with the FtsZ membrane anchor SepF as a bait for co-immunoprecipitation (co-IP) of divisome members. For SepF interactome we carried out the co-IPs using anti-Scarlet antibodies in Cglu strains expressing either SepF-mScarlet or mScarlet. Co-IPs using anti-SepF were also performed from Cglu or SepF-mScarlet strains. GLP interactomes were analysed in two strains: Cglu and Cglu_Dglpr strains.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Corynebacterium Glutamicum Atcc 13032

SUBMITTER: Alejandro Leyva

LAB HEAD: Rosario Durán

PROVIDER: PXD037255 | Pride | 2023-07-26

REPOSITORIES: Pride

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Two elongasomes generate rod shape in a unicellular microorganism

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Solar simulated ultraviolet radiation induces histone 3 methylation changes in the gene promoters of matrix metalloproteinases 1 and 3 in primary human dermal fibroblasts

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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