Proteomic analysis of H460 and MCF7 cell lines grown in 2D and 3D culture conditions

ABSTRACT: In this study we analyzed the proteomic profiles of H460, and MCF7 3D tumor spheroids grown either in standard medium (3D_SM), or in FBSlow conditions (3D_FBSlow) and compared them to their relative 2D cultures (2D). Standard medium consisted of DMEM/F-12 supplemented with 0.5% Glucose, 2.5mM L-Glutamine, 2% B-27, 5μg/ml Heparin, 20μg/ml Insulin, 20ng/ml EGF, 20ng/ml Recombinant Human bFGF, 0.1% Bovine Serum Albumin (BSA) and 1% (v/v) of penicillin/streptomycin 100U/ml. FBSlow medium consisted of customized nutrient-restricted RPMI or DMEM culture media supplemented with only 2% FBS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Lung Cancer,Breast Cancer

SUBMITTER: Marco Gaspari  

LAB HEAD: Marco Gaspari

PROVIDER: PXD037256 | Pride | 2025-01-20

REPOSITORIES: Pride