Dataset Information

PRM-based quantitative proteomic analysis of ribosomes in GC-1 cell line

ABSTRACT: Relative protein quantification of RPL4 and RPL39L in non-ribosome, 40S, 60S and ribosome fractions by PRM;Absolute quantification of RPL39 and RPL39L levels in ribosomes of GC-1 wild type cell, and RPL39L-IRES-eGFP-overexpressed RibosomeCore-/Y GC-1 cells with or without dox-induced expression of RPL39 by PRM. Bovine serum albumin (BSA) as a negative control.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: yue wang

LAB HEAD: Xuejiang Guo

PROVIDER: PXD037257 | Pride | 2022-10-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	GC-1-Ribosome-WT-Lenti-Dox-RPL39_39L-20211228.zip	Other
	PRM_RPL39_39L_5fmol_500ng_GC-1_Lenti-Dox_Ribo_rep1_20211227.raw	Raw
	PRM_RPL39_39L_5fmol_500ng_GC-1_Lenti-Dox_Ribo_rep2_20211227.raw	Raw
	PRM_RPL39_39L_5fmol_500ng_GC-1_Lenti-Dox_Ribo_rep3_20211227.raw	Raw
	PRM_RPL39_39L_5fmol_500ng_GC-1_Lenti_Dox_Ribo_rep1_20211227.raw	Raw

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Publications

A male germ-cell-specific ribosome controls male fertility.

Li Huiling H Huo Yangao Y He Xi X Yao Liping L Zhang Hao H Cui Yiqiang Y Xiao Huijuan H Xie Wenxiu W Zhang Dejiu D Wang Yue Y Zhang Shu S Tu Haixia H Cheng Yiwei Y Guo Yueshuai Y Cao Xintao X Zhu Yunfei Y Jiang Tao T Guo Xuejiang X Qin Yan Y Sha Jiahao J

Nature 20221214 7941

Ribosomes are highly sophisticated translation machines that have been demonstrated to be heterogeneous in the regulation of protein synthesis1,2. Male germ cell development involves complex translational regulation during sperm formation3. However, it remains unclear whether translation during sperm formation is performed by a specific ribosome. Here we report a ribosome with a specialized nascent polypeptide exit tunnel, RibosomeST, that is assembled with the m ...[more]

PMID: 36517592

Dataset Information

PRM-based quantitative proteomic analysis of ribosomes in GC-1 cell line

Dataset's files

Publications

A male germ-cell-specific ribosome controls male fertility.

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