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Metaproteomic analysis of dichloromethane-fermenting enrichment culture DFE

ABSTRACT: Enrichment culture DFE is capable of fermenting the toxic pollutant dichloromethane (DCM; CH2Cl2) into environmentally benign acetate. The dominant organism in the enrichment culture is a novel bacterium in the Peptococcaceae family, “Candidatus Formimonas warabiya” strain DCMF. This metaproteomic study analysed culture DFE during growth with four substrates – DCM, glycine betaine, choline, and methanol – all of which are primarily, if not solely, metabolised by strain DCMF.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Candidatus Formimonas Warabiya

SUBMITTER: Sophie Holland

LAB HEAD: Michael Manefield

PROVIDER: PXD037334 | Pride | 2023-03-11

REPOSITORIES: Pride

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Metaproteomics reveals methyltransferases implicated in dichloromethane and glycine betaine fermentation by 'Candidatus Formimonas warabiya' strain DCMF.

Holland Sophie I SI Vázquez-Campos Xabier X Ertan Haluk H Edwards Richard J RJ Manefield Michael J MJ Lee Matthew M

Frontiers in microbiology 20221207

Dichloromethane (DCM; CH2Cl2) is a widespread pollutant with anthropogenic and natural sources. Anaerobic DCM-dechlorinating bacteria use the Wood-Ljungdahl pathway, yet dechlorination reaction mechanisms remain unclear and the enzyme(s) responsible for carbon-chlorine bond cleavage have not been definitively identified. Of the three bacterial taxa known to carry out anaerobic dechlorination of DCM, 'Candidatus Formimonas warabiya' strain DCMF is the only organism th ...[more]

PMID: 36569084

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