Dataset Information

Proteomics reveals multiple effects of titanium dioxide and silver nanoparticles in the metabolism of turbot, Scophthalmus maximus

ABSTRACT: This project aimed to assess the toxicity underlying the exposure of Turbot (Scophthalmus maximus), an economically valuable fish species, to TiO2 and Ag nanoparticles (NPs). To carry out the study, juvenile fish were exposed to TiO2 and Ag NPs incorporated in the diet. After 14 days of exposure fish were euthanized and liver and kidney samples collected for proteomics. The shotgun proteomics analysis revealed quantitative changes in multiple proteins. The results suggest effects of TiO2 and Ag NPs in energy/lipid metabolism and for instance alterations in ribosome functions, protein biosynthesis and related processes. This proteomic study provided as well candidate biomarkers for NPs exposure in aquaculture species.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive HF

ORGANISM(S): Scophthalmus Maximus

TISSUE(S): Liver, Kidney

SUBMITTER: Alexandre Campos

LAB HEAD: Alexandre Campos

PROVIDER: PXD037420 | Pride | 2022-10-18

REPOSITORIES: Pride

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Publications

Proteomics reveals multiple effects of titanium dioxide and silver nanoparticles in the metabolism of turbot, Scophthalmus maximus.

Araújo Mário J MJ Sousa Maria L ML Fonseca Elza E Felpeto Aldo Barreiro AB Martins José Carlos JC Vázquez María M Mallo Natalia N Rodriguez-Lorenzo Laura L Quarato Monica M Pinheiro Ivone I Turkina Maria V MV López-Mayán Juan José JJ Peña-Vázquez Elena E Barciela-Alonso María Carmen MC Spuch-Calvar Miguel M Oliveira Miguel M Bermejo-Barrera Pilar P Cabaleiro Santiago S Espiña Begoña B Vasconcelos Vitor V Campos Alexandre A

Chemosphere 20220822 Pt 1

Titanium dioxide (TiO<sub>2</sub>) and silver (Ag) NPs are among the most used engineered inorganic nanoparticles (NPs); however, their potential effects to marine demersal fish species, are not fully understood. Therefore, this study aimed to assess the proteomic alterations induced by sub-lethal concentrations citrate-coated 25 nm ("P25") TiO<sub>2</sub> or polyvinylpyrrolidone (PVP) coated 15 nm Ag NPs to turbot, Scophthalmus maximus. Juvenile fish were exposed to the NPs through daily feedin ...[more]

PMID: 36007739

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Project description:An estimated 1% or less of the nanoparticles (NPs) deposited in lungs translocate to systemic circulation and enter other organs; however this estimation may not be accurate considering the low sensitivity of the existing in vivo NP detection methods. Moreover, the biological outcomes of such low levels of translocation are not elucidated. The objectives of the present study were to employ a Nano-scale Hyperspectral Microscope to spatially observe and spectrally profile NPs in tissues, and characterize the effects of NPs in blood, liver and heart following pulmonary deposition and subsequent translocation from lungs. Adult female C57BL/6 mice were exposed via intratracheal instillation to 18 and 162 µg per mouse of industrially relevant non-doped titanium dioxide nanoparticles (nano-TiO2). Using the Nano-scale Hyperspectral Microscope translocation to heart and liver was confirmed at both doses and to blood at the highest dose at 24 hours post-exposure time-point. The analysis of biological effects using DNA microarrays, RT-qPCR and ELISA revealed activation of complement cascade and inflammatory process in heart and specific activation of complement factor 3 in blood, potentially suggestive of activation of early innate immune response essential for particle opsonisation and clearance. The liver showed subtle response with changes in the expression of few genes associated with acute phase genes. This study establishes a direct link between particle translocation and systemic effects. An estimated 1% or less of the nanoparticles (NPs) deposited in lungs translocate to systemic circulation and enter other organs; however this estimation may not be accurate considering the low sensitivity of the existing in vivo NP detection methods. Moreover, the biological outcomes of such low levels of translocation are not elucidated. The objectives of the present study were to employ a Nano-scale Hyperspectral Microscope to spatially observe and spectrally profile NPs in tissues, and characterize the effects of NPs in blood, liver and heart following pulmonary deposition and subsequent translocation from lungs. Adult female C57BL/6 mice were exposed via intratracheal instillation to 18 and 162 µg per mouse of industrially relevant non-doped titanium dioxide nanoparticles (nano-TiO2). Using the Nano-scale Hyperspectral Microscope translocation to heart and liver was confirmed at both doses and to blood at the highest dose at 24 hours post-exposure time-point. The analysis of biological effects using DNA microarrays, RT-qPCR and ELISA revealed activation of complement cascade and inflammatory process in heart and specific activation of complement factor 3 in blood, potentially suggestive of activation of early innate immune response essential for particle opsonisation and clearance. The liver showed subtle response with changes in the expression of few genes associated with acute phase genes. This study establishes a direct link between particle translocation and systemic effects. This experiment consists of one dose of nano-TiO2 (162 ug) and one control. There are 2 time points for each treatment and control group, e.g., day 1 and day 28. Each dose or time point has 5-6 biological replicates. There are total 22 samples (arrays)

Dataset Information

Proteomics reveals multiple effects of titanium dioxide and silver nanoparticles in the metabolism of turbot, Scophthalmus maximus

Publications

Proteomics reveals multiple effects of titanium dioxide and silver nanoparticles in the metabolism of turbot, Scophthalmus maximus.

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