Exploring Alternative Cysteine-reactive Chemistry for MS-cleavable Cross-linking Mass Spectrometry

ABSTRACT: To enable global PPI mapping, we have explored an alternative cysteine labeling chemistry, and thus designed and synthesized a sulfoxide-containing MS-cleavable haloacetamide-based cross-linker, DiBromoAcetamide SulfOxide (DBrASO). In combination with a newly developed SEC-HpHt fractionation method, we have successfully applied DBrASO for cross-linking of HEK293 cell lysates and demonstrated its capability to complement lysine-reactive reagents in order to expand PPI coverage at systems-level.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: Fenglong Jiao  

LAB HEAD: Lan Huang

PROVIDER: PXD037443 | Pride | 2023-07-20

REPOSITORIES: Pride