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Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates

ABSTRACT: Ectopic expression in fibroblasts of synapsin 1 and synaptophysin is sufficient to generate condensates of vesicles highly reminiscent of synaptic vesicle (SV) clusters and with liquid-like properties. We show that unlike synaptophysin, other major integral SV membrane proteins fail to form condensates with synapsin, but coassemble into the clusters formed by synaptophysin and synapsin in this ectopic expression system. Another vesicle membrane protein, ATG9A, undergoes activity-dependent exo-endocytosis at synapses, raising questions about the relation of ATG9A traffic to the traffic of SVs. We have found that both in fibroblasts and in nerve terminals ATG9A does not coassemble into synaptophysin-positive vesicle condensates but localizes on a distinct class of vesicles that also assembles with synapsin but into a distinct phase. Our findings suggest that ATG9A undergoes differential sorting relative to SV proteins and also point to a dual role of synapsin in controlling clustering at synapses of SVs and ATG9A vesicles

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Cercopithecus Aethiops (green Monkey) (grivet)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Zon Weng Lai

LAB HEAD: Pietro De Camilli

PROVIDER: PXD037490 | Pride | 2022-12-24

REPOSITORIES: Pride

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