Dataset Information

A small CRISPR/Cas-associated RNA regulates ferrous iron transport in Neisseria meningitidis

ABSTRACT: In this study the targetome of the endonuclease Cas9 and a CRISPR-associated small RNA (scaRNA/NMnc0040) was determined in Neisseria meninigitidis serogroup C strain 8013 by quantitative proteomics.

INSTRUMENT(S):

ORGANISM(S): Neisseria Meningitidis Serogroup C (strain 8013)

TISSUE(S): Cell Culture

SUBMITTER: Denise Müller

LAB HEAD: Christoph Schoen

PROVIDER: PXD037567 | Pride | 2026-08-07

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	2020-03-25_velos_22671.raw	Raw
	2020-03-25_velos_22672.raw	Raw
	2020-03-25_velos_22673.raw	Raw
	2020-03-25_velos_22674.raw	Raw
	2020-03-25_velos_22675.raw	Raw

Items per page:

1 - 5 of 137

Publications

The CRISPR/Cas-associated scaRNA modulates efeUOB expression and stress responses in Neisseria meningitidis.

Pytlik Denise D Gerovac Milan M Bischler Thorsten T Schlosser Andreas A Vogel Jörg J Schoen Christoph C

microLife 20260720

Neisseria meningitidis is a human-adapted commensal pathogen that must continuously balance nutrient acquisition with stress tolerance. Here, we identify a type II-C CRISPR/Cas-associated small RNA (scaRNA) as a posttranscriptional regulator of the efeUOB operon and oxidative stress responses. Using in vitro RNA binding and structure probing assays, we show that the scaRNA interacts with the 5' untranslated region of efeO mRNA, leading to reduced translation of this c ...[more]

PMID: 42549139

Similar Datasets

Project description:Many of the enzymes involved in xenobiotic metabolism are maintained at a low basal level and are only synthesized in response to activation of upstream sensor/effector proteins. This induction can have implications in a variety of contexts, particularly during the study of the pharmacokinetics, pharmacodynamics and drug-drug interaction profile of a candidate therapeutic compound. Previously, we combined in vivo SILAC material with a targeted high resolution single ion monitoring (tHR/SIM) LC-MS/MS approach for quantification of 197 peptide pairs, representing 51 drug metabolism enzymes (DME), in mouse liver. However, as important enzymes (for example, cytochromes P450 (Cyp) of the 1a and 2b subfamilies) are maintained at low or undetectable levels in the liver of unstimulated metabolically-labelled mice, quantification of these proteins was unreliable. In the present study, we induced DME expression in labelled mice through synchronous ligand-mediated activation of multiple upstream nuclear receptors, thereby enhancing signals for proteins including Cyps 1a, 2a, 2b, 2c and 3a. With this enhancement, 115 unique, lysine-containing, Cyp-derived peptides were detected in the liver of a single animal, as opposed to 56 in a pooled sample from three uninduced animals. A total of 386 peptide pairs were quantified by tHR/SIM, representing 68 Phase I, 30 Phase II and 8 control proteins. This method was employed to quantify changes in DME expression in the hepatic cytochrome P450 reductase null (HRN) mouse. We observed compensatory induction of several enzymes, including Cyps 2b10, 2c29, 2c37, 2c54, 2c55, 2e1, 3a11 and 3a13, carboxylesterase (Ces) 2a and glutathione S-transferases (Gst) m2 and m3, along with down-regulation of hydroxysteroid dehydrogenases (Hsd) 11b1 and 17b6. Using DME-enhanced in vivo SILAC material with tHR/SIM, therefore, permits the robust analysis of multiple DME of import to xenobiotic metabolism, with improved utility for the study of drug pharmacokinetics, pharmacodynamics, and of chemically-treated and genetically-modified mouse models.

Dataset Information

A small CRISPR/Cas-associated RNA regulates ferrous iron transport in Neisseria meningitidis

Dataset's files

Publications

The CRISPR/Cas-associated scaRNA modulates <i>efeUOB</i> expression and stress responses in <i>Neisseria meningitidis</i>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

A small CRISPR/Cas-associated RNA regulates ferrous iron transport in Neisseria meningitidis

Dataset's files

Publications

The CRISPR/Cas-associated scaRNA modulates &lt;i&gt;efeUOB&lt;/i&gt; expression and stress responses in &lt;i&gt;Neisseria meningitidis&lt;/i&gt;.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

The CRISPR/Cas-associated scaRNA modulates <i>efeUOB</i> expression and stress responses in <i>Neisseria meningitidis</i>.