Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysing the effects of glycosylation knockdown via CRISPR interference in Burkholderia thailandensis

ABSTRACT: To determine the effect of CRISPR interference-mediated silencing of glycosylation in Burkholderia thailandensis harbouring a pglL-targeted guide, and a nontarget guide using Data Independent Acquisition

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Burkholderia Thailandensis E264

SUBMITTER: Jessica Lewis

LAB HEAD: Nichollas Scott

PROVIDER: PXD037595 | Pride | 2023-03-20

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Similar Datasets

ii. Analysing the effects of glycosylation knockdown via CRISPR interference in Burkholderia multivorans

Project description:To determine the effect of CRISPR interference-mediated silencing of glycosylation in Burkholderia multivorans harbouring a pglL-targeted guide, and a nontarget guide using Data Independent Acquisition

2023-03-20 | PXD037585 | Pride

Analysing the glycoproteome of Burkholderia thailandensis following knockdown of glycosylation using CRISPR interference

Project description:To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia thailandensis through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment. Using a pglL (BTH_I0650)-targeting guide, and a non-target guide.

2023-03-20 | PXD037773 | Pride

Analysing the effects of glycosylation knockdown via CRISPR interference in Burkholderia diffusa

Project description:To determine the affect of CRISPR interference-mediated silencing of glycosylation in Burkholderia diffusa harbouring a pglL (WL85_RS02125)-targeted guide, and a nontarget guide using Data Independent Acquisition

2023-03-20 | PXD037556 | Pride

Validating the use of CRISPR interference for the modulation of glycosylation in Burkholderia thailandensis

Project description:To determine whether CRISPR interference can be used to modulate glycosylation in Burkholderia thailandensis

2023-03-20 | PXD036623 | Pride

Identifying the glycoproteome of Burkholderia thailandensis following knockdown of glycosylation using CRISPR interference - loading control for glycopeptide enrichment

2023-03-20 | PXD037748 | Pride

Analysing the glycoproteome of Burkholderia cenocepacia following knockdown of glycosylation using CRISPR interference

Project description:To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia cenocepacia through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment. Using a pglL (BCAL0960)-targeting guide, and a non-target guide.

2023-03-20 | PXD037778 | Pride

Direct comparison between a Burkholderia cenocepacia glycosylation mutant, and a CRISPRi-mediated glycosylation knockdown strain

Project description:To determine whether CRISPR interference can be used to recapitulate a glycosylation null mutant strain in Burkholderia cenocepacia via data independent acquisition mass spectrometry using a low level of dCas9 induction. A guideless plasmid was containing in the wildtype and pglL mutant strains, and the strains harbouring the dCas9 insertion containing a pglL-targeting plasmid Bc-pglL-C, non-target, and the guideless plasmid.

2023-03-20 | PXD038043 | Pride

Validating the use of CRISPR interference for the modulation of glycosylation in Burkholderia diffusa

Project description:To determine whether CRISPR interference can be used to silence the expression of pglL in Burkholderia diffusa and therefore modulate glycosylation

2023-03-20 | PXD036585 | Pride

Identifying the glycoproteome of Burkholderia cenocepacia following knockdown of glycosylation using CRISPR interference (low dCas9 induction) - loading control for glycopeptide enrichment

Project description:To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia cenocepacia through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment with 0.1% rhamnose for dCas9 induction– loading control pre-enrichment. Using strains harbouring the pglL-targeting guide C (Bc-pglL-C) and guideless plasmids.

2023-03-20 | PXD038044 | Pride

Identifying the glycoproteome of Burkholderia cenocepacia following knockdown of glycosylation using CRISPR interference (low dCas9 induction)

Project description:To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia cenocepacia through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment with 0.1% rhamnose for dCas9 induction. Using strains harbouring the pglL-targeting guide C (Bc-pglL-C) and guideless plasmids.

2023-03-20 | PXD040181 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data