Proteomics analysis of zebrafish spinal cord regeneration following extracellular matrix manipulations

ABSTRACT: Label-free mass spectrometry-based quantitative proteomics was applied to a larval zebrafish spinal cord injury model, which allows axon regeneration and functional recovery within two days (days post lesion; dpl) after a spinal cord transection in 3 day-old larvae (dpf). Proteomic profiling was performed of the lesion site at 1 dpl in control animals and animals with pdgfrb+ cell-specific overexpression of either zebrafish chondoradherin (chad; chad-mCherry fusion), fibromodulin a (fmoda; fmoda-mCherry fusion), lumican (lum; lum-mCherry fusion) or prolargin (prelp; prelp-mCherry fusion).

INSTRUMENT(S):

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Spinal Cord

SUBMITTER: Barbara Steigenberger  

LAB HEAD: Dr. Daniel Wehner

PROVIDER: PXD037605 | Pride | 2023-09-06

REPOSITORIES: Pride