RBPMS mediates smooth muscle specific alternative splicing via oligomerization and modulation of trans splicing factor recruitment

ABSTRACT: RBPMS regulates alternative splicing decision via recruiting other trans factors. This experiment aims to investigate the interactome of Strep-II tagged recombinant RBPMS in Hela nuclear extract. The interactome of the full length protein is compared to the construct lacking the far C-terminal 20 amino acids. The “R” series indicates full-length RBPMS pull-down. The “P” series indicates truncated RBPMS pull-down. The “BL” series indicates full length RBPMS pull-down in the absence of nuclear extract input but with bovine serum albumin beads blocking reagent. The “N” series indicates non-baited negative control pull-down.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Yi Yang  

LAB HEAD: Chris WJ Smith

PROVIDER: PXD037620 | Pride | 2023-10-24

REPOSITORIES: Pride