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Immunopeptidomics of a brain tumour cell line to identify HLA presented Zika peptides

ABSTRACT: We present data of a brain tumour cell line (USP7) infected with Zika virus (Strain: BeH819966, GenBank: KU365779.1) to identify CD8 T cell epitopes eluted from the cell surface using mass spectrometry (immunopeptidomics) of presented HLA bound Zika peptides.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Cell Culture

DISEASE(S): Zika Virus Congenital Syndrome

SUBMITTER: Alistair Bailey

LAB HEAD: Paul J Skipp

PROVIDER: PXD037627 | Pride | 2025-11-10

REPOSITORIES: Pride

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			Action	DRS
	ZIKASept20_Sample10d.mgf	Mgf
	ZIKASept20_Sample10d.raw	Raw
	ZIKASept20_Sample11d.mgf	Mgf
	ZIKASept20_Sample11d.raw	Raw
	ZIKASept20_Sample12d.mgf	Mgf

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1 - 5 of 53

Publications

Identification of natural Zika virus peptides presented on the surface of paediatric brain tumour cells by HLA class I.

Sherwood Matt M Nicholas Ben B Bailey Alistair A Mitsugi Thiago Giove TG Kaid Carolini C Okamoto Oswaldo K OK Skipp Paul P Ewing Rob M RM

PloS one 20251030 10

Despite decades of research, survival from brain cancer has scarcely improved and is drastically lower than that of other cancers. Novel therapies, such as immunotherapy, hold great promise for treating brain tumours and are desperately needed. Zika virus (ZIKV) infects and kills aggressive cancer cells with stem-like properties (CSCs) from both paediatric and adult brain tumours. Whilst T cell recruitment into ZIKV-infected brain tumours is becoming well documented, the specific mechanisms thro ...[more]

PMID: 41166287

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Project description:We present data comparing an immortal macrophage-like cell lines with ex-vivo infected lung tissues as the presentation models of direct observation of CD4 and CD8 T cell epitopes eluted from the cell surface using mass spectrometry (immunopeptidomics) of presented HLA bound peptides.

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Identification of sensitiser modified HLA-peptides presented by keratinocytes

Project description:Skin sensitisation following the covalent modification of proteins by low molecular weight chemicals (haptenation) is mediated by cytotoxic T lymphocyte (CTL) recognition of human leukocyte antigen molecules (HLA) presented on the surface of almost all nucleated cells. There are three nonmutually exclusive models for how haptens mediate CTL recognition: direct stimulation by haptenated peptides; hapten modification of HLA leading to an altered HLA-peptide repertoire; or a hapten altered proteome leading to an an altered HLA-peptide repertoire. To address the mechanism in skin, we set out to utilise proteomic analysis of keratinocyte presented antigens following exposure to 2,4-dinitrochlorobenzene (DNCB). We show that following DNCB exposure, cultured keratinocytes present cysteine haptenated (dinitrophenylated) peptides in multiple HLA molecules. In addition, we find that one of the DNCB modified peptides derives from the active site of cytosolic glutathione-S transferase-ω. These results provide support for the hypothesis that a key mechanism of skin sensitisation is stimulation of CTLs by haptenated peptides.

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Identification of neoantigens in oesophageal adenocarcinoma

Project description:We present data from tissues of seven oesophageal adenocarcinomas of CD4 and CD8 T cell epitopes eluted from the cell surface using mass spectrometry (immunopeptidomics) of presented HLA bound peptides.This dataset forms part of the publication:Nicholas, B., Bailey, A., McCann, K.J., Wood, O., Walker, R.C., Parker, R., Ternette, N., Elliott, T., Underwood, T.J., Johnson, P. and Skipp, P. (2022), Identification of neoantigens in esophageal adenocarcinoma. Immunology. Accepted Author Manuscript. https://doi.org/10.1111/imm.13578

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