An Optimized Suspension Trapping (S-Trap) Method for Phosphoproteomics Sample Preparation

ABSTRACT: The performance of the S-Trap protocol for phosphoproteomics studies for proteome and phosphoproteome was systematically evaluated in big-scale and small-scale samples. The results showed the existing protocol was problematic for phsophopeptides enrichment. After employing optimized S-Trap methodology, more peptides and phosphopeptides are found in the application for extracellular vesicles.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Fujia Wang  

LAB HEAD: Maarten Altelaar

PROVIDER: PXD037751 | Pride | 2023-06-19

REPOSITORIES: Pride