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Identification of proximal interactome of c17orf80 C- and N-terminally tagged with BirA*

ABSTRACT: To validate the interaction of c17orf80 with mitochondrial nucleoid, we used proximity-labelling proteomics. We tagged c17orf80 with BirA* from either C- or N-terminus and expressed the fusion proteins in FlpIN-TRex 293 cell line. As controls for endogenous biotinylation, non-induced cells were used.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Alisa Potter

LAB HEAD: Hans Spelbrink

PROVIDER: PXD037857 | Pride | 2023-07-11

REPOSITORIES: Pride

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