Proteomics

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Testis-specific serine kinase 3 is required for sperm morphogenesis and male fertility


ABSTRACT: TSSK3 is expressed in elongating sperm and localizes to the sperm tail. To define the essential roles of TSSK3 in vivo and elucidate the mechanisms causing the phenotype, we conducted phosphoproteomics using purified germ cells from Tssk3 HET and KO testes.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: Antrix Jain  

LAB HEAD: Martin M. Matzuk

PROVIDER: PXD038012 | Pride | 2023-11-21

REPOSITORIES: Pride

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Publications

Testis-specific serine kinase 3 is required for sperm morphogenesis and male fertility.

Nozawa Kaori K   Garcia Thomas X TX   Kent Katarzyna K   Leng Mei M   Jain Antrix A   Malovannaya Anna A   Yuan Fei F   Yu Zhifeng Z   Ikawa Masahito M   Matzuk Martin M MM  

Andrology 20221116 5


<h4>Background</h4>The importance of phosphorylation in sperm during spermatogenesis has not been pursued extensively. Testis-specific serine kinase 3 (Tssk3) is a conserved gene, but TSSK3 kinase functions and phosphorylation substrates of TSSK3 are not known.<h4>Objective</h4>The goals of our studies were to understand the mechanism of action of TSSK3.<h4>Materials and methods</h4>We analyzed the localization of TSSK3 in sperm, used CRISPR/Cas9 to generate Tssk3 knockout (KO) mice in which nea  ...[more]

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