Proteomics

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Direct comparison between a Burkholderia cenocepacia glycosylation mutant, and a CRISPRi-mediated glycosylation knockdown strain

ABSTRACT: To determine whether CRISPR interference can be used to recapitulate a glycosylation null mutant strain in Burkholderia cenocepacia via data independent acquisition mass spectrometry using a low level of dCas9 induction. A guideless plasmid was containing in the wildtype and pglL mutant strains, and the strains harbouring the dCas9 insertion containing a pglL-targeting plasmid Bc-pglL-C, non-target, and the guideless plasmid.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Burkholderia Cenocepacia K56-2valvano

SUBMITTER: Jessica Lewis  

LAB HEAD: Nichollas Scott

PROVIDER: PXD038043 | Pride | 2023-03-20

REPOSITORIES: Pride

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