Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mapping Ndc80 phosphorylation sites

ABSTRACT: Aurora A-TPX2 activity limits NDC80 phosphorylation

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Katarzyna Kowalczyk

LAB HEAD: Francis A Barr

PROVIDER: PXD038197 | Pride | 2023-03-06

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Phospho__STY_Sites.txt	Txt
	QEX02_KMK_221013_G1_Ndc80_DMSO_IP_total_085.raw	Raw
	QEX02_KMK_221013_G1_Ndc80_DMSO_phospho_081.raw	Raw
	QEX02_KMK_221013_G2_Ndc80_AurA_IP_total_086.raw	Raw
	QEX02_KMK_221013_G2_Ndc80_AurA_phospho_082.raw	Raw

Items per page:

1 - 5 of 28

Similar Datasets

Higher-order assembly is a regulatory switch that promotes outer kinetochore recruitment

Project description:To faithfully segregate chromosomes during vertebrate mitosis, kinetochore-microtubule interactions must be restricted to a single site on each chromosome. Prior work on pair-wise kinetochore protein interactions has been unable to identify the mechanisms that prevent kinetochore formation in regions with a low density of CENP-A nucleosomes. To investigate the impact of higher-order assembly on kinetochore formation, we generated defined oligomers of the inner kinetochore protein CENP-T using two distinct, genetically engineered systems in human cells. Although individual CENP-T molecules interact poorly with other kinetochore proteins, oligomers that mimic the centromeric density of CENP-T trigger the robust formation of functional, cytoplasmic kinetochore-like particles. Both in cells and in vitro, each molecule of oligomerized CENP-T recruits substantially higher levels of outer kinetochore components than monomeric CENP-T molecules. Thus, the density-dependence of CENP-T restricts outer kinetochore recruitment to centromeres, where densely packed CENP-A recruits a high local concentration of CENP-T.

2023-11-09 | PXD042174 | Pride

TPX2-mediated autophagy maintains cancer stemness in LUAD

Project description:Targeting protein for Xklp2 (TPX2) is a mitotic regulator frequently overexpressed in lung adenocarcinoma (LUAD), while its role in cancer stemness and autophagy remains incompletely understood. In this study, integrative bioinformatic analyses based on TCGA and GTEx datasets revealed that TPX2 overexpression in LUAD was associated with poor prognosis, elevated stemness scores, and increased expression of cancer stem cell-related markers. To investigate the molecular mechanisms underlying TPX2-mediated regulation in LUAD, RNA sequencing was performed in PC9 cells following TPX2 knockdown. Transcriptomic profiling and pathway analyses identified significant alterations in autophagy-related signaling pathways. In parallel, co-immunoprecipitation coupled with mass spectrometry (IP-MS) was conducted to characterize TPX2-associated protein interactions and explore potential regulatory networks involved in autophagy. Functional experiments further demonstrated that TPX2 depletion impaired autophagic flux and reduced stemness-associated phenotypes in LUAD cells, whereas TPX2 overexpression exerted opposite effects. These data provide transcriptomic and proteomic resources for investigating the role of TPX2-mediated autophagy in LUAD progression and cancer stemness.

2026-05-28 | GSE333531 | GEO

Array-based Comparative Genomic Hybridization of Melanoma Cell Lines

2012-08-27 | GSE38946 | GEO

Project description:NDC80 in Plasmodium Chromosome Segregation

| PRJNA637024 | ENA

Array-based Comparative Genomic Hybridization of Melanoma Cell Lines

Project description:This study explored the role of targeting protein for Xklp2 (TPX2), located with 20q11.2, in melanoma pathogenesis, based on initial findings that it was commonly gained and overexpressed. TPX2 copy number was evaluated in melanoma cell lines compared to control DNA. Thrity-three cell lines were analyzed by Agilent SurePrint G3 Human CGH Microarray 2x400K. Samples were compared to Human Female DNA from Promega.

2012-08-27 | E-GEOD-38946 | biostudies-arrayexpress

PARP1 proximity proteomics reveals interaction partners at stressed replication forks

Project description:PARP1 mediates poly-ADP-ribosylation of proteins on chromatin in response to different types of DNA lesions. PARP inhibitors are used for the treatment of BRCA1/2-deficient breast, ovarian, and prostate cancer. Loss of DNA replication fork protection is proposed as one mechanism that contributes to the vulnerability of BRCA1/2-deficient cells to PARP inhibitors. However, the mechanisms that regulate PARP1 activity at stressed replication forks remain poorly understood. Here, we performed proximity proteomics of PARP1 and isolation of proteins on stressed replication forks to map putative PARP1 regulators. We identified TPX2 as a direct PARP1-binding protein that regulates the auto-ADP-ribosylation activity of PARP1. TPX2 interacts with DNA damage response proteins and promotes homology-directed repair of DNA double-strand breaks. Moreover, TPX2 mRNA levels are increased in BRCA1/2-mutated breast and prostate cancers, and high TPX2 expression levels correlate with the sensitivity of cancer cells to PARP-trapping inhibitors. We propose that TPX2 confers a mitosis-independent function in the cellular response to replication stress by interacting with PARP1.

2022-10-11 | PXD037154 | Pride

Transcription profiling of human pulp and odontoblasts reponse to TGF-beta1

Project description:Transforming growth factor beta 1 (TGF-Î²1) is the most extensively studied growth factor in dentin-pulp complex, with pleiotropic effects on pulp response and healing. Our main objective was to analyze the expression profile of pulp tissue and odontoblasts, and the effects of TGF-Î²1 on these profiles in cultured human pulp and odontoblasts with a specific interest in the anti- and pro-inflammatory cytokines. Experiment Overall Design: Pulp tissues and odontoblasts were cultured for different time periods, and microarray was performed to both cultured and native samples to detect the effects of TGF-Î²1. Expression of various interleukins (IL) were confirmed by RT-PCR, and in +/- TGF-Î²1 treated pulps also by antibody array.

2008-06-16 | E-GEOD-8730 | biostudies-arrayexpress

Cytokinetic Abscission in Toxoplasma gondii is governed by Protein Phosphatase 2A and the Daughter Cell Scaffold Complex

Project description:Cytokinetic abscission is the last step of cell division during which the daughter cells physically separate through the generation of barriers in the form of new plasma membrane or cell wall. While the contractile ring is a prominent structure during cytokinesis in bacteria, fungi and animal cells, the mechanism is divergent in Apicomplexa. In Toxoplasma gondii, two daughter cells are formed within the intact mother cell by endodyogeny. The acquisition of plasma membrane at the end of the division cycle occurs by an unknown mechanism, when the mother cell disassembles, and the daughter cells emerge. Here we identified and functionally characterized the essential subunits of a predicted PP2A complex in T. gondii, named PP2A-2. The three subunits PP2A-A2, -B2 and -C2 exhibit a dynamic localization during parasite division. Their individual downregulation prevents the accumulation of plasma membrane at the division plane, resulting in a block of cytokinesis. Remarkably, the absence of cytokinetic abscission does not preclude division cycles and the consecutive progeny is able to successfully egress from the infected cells but fails to glide andinvade new host cells.

2024-06-13 | PXD051495 | Pride

RNA-seq to investigate gene expression profiles in tumor infiltrating CD8+ T cells with TPX2-overexpression

Project description:CD8+ T cells were isolated from fresh human HCC tissues, then treated with lentivirus for TPX2 over-expression or control

2024-02-15 | E-MTAB-10040 | biostudies-arrayexpress

Gene expression analysis of MTBTOM tumors

Project description:Tumors that overexpress the MYC oncogene frequently demonstrate aneuploidy, numerical chromosome alterations associated with highly aggressive cancers, rapid tumor evolution, and poor patient outcome. Here, we identify that MYC overexpression induces defects in microtubule nucleation and mitotic spindle assembly, promoting chromosome segregation defects, micronuclei and chromosomal instability (CIN). High TPX2 expression is permissive for mitotic spindle assembly and chromosome segregation in cells with MYC overexpression; whereas TPX2 depletion blocks mitotic progression, induces cell death and prevents tumor growth. Attenuating MYC expression reverses mitotic defects, even in established tumor cell lines, implicating an ongoing role for high MYC in the persistence of CIN in tumors. Our studies implicate the MYC oncogene as a regulator of spindle assembly and identify a new MYC-TPX2 synthetic-lethal interaction that could represent a future therapeutic strategy in MYC-overexpressing cancers. Moreover, our studies suggest that blocking MYC activity can attenuate the emergence of CIN and tumor evolution.

2020-03-30 | GSE130921 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data