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The global downregulation of protein synthesis observed during hepatogenic maturation is associated with a decrease in TOP mRNA translation The global downregulation of protein synthesis observed during hepatogenic maturation is associated with a decrease in TOP mRNA translation

ABSTRACT: Translational regulation is of paramount importance for proteome remodeling during stem cell differentiation both at the global and transcript-specific levels. In this study, we characterized translational remodeling during hepatogenic differentiation of induced pluripotent stem cells (iPSCs) by polysome profiling. We demonstrate that protein synthesis increases during exit from pluripotency, and is then globally repressed during later steps of hepatogenic maturation. This global downregulation of translation is accompanied by a decrease in the protein abundance of components of the translation machinery, which involves a global reduction in translational efficiency of terminal oligopyrimidine tract (TOP) mRNA encoding translation-related factors. Despite global translational repression during hepatogenic differentiation, key hepatogenic genes remain efficiently translated, and the translation of several transcripts involved in hepato-specific functions and metabolic maturation are even induced. We conclude that, during hepatogenic differentiation, a global decrease in protein synthesis is accompanied by a specific translational rewiring of hepato-specific transcripts.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Pluripotent Stem Cell, Hepatocyte

SUBMITTER: Marc Dieu

LAB HEAD: Prof Patricia Renard

PROVIDER: PXD038204 | Pride | 2022-12-26

REPOSITORIES: Pride

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