Proteomics

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Precise diagnosis and typing of early-stage renal immunoglobulin-derived amyloidosis by label-free quantification of parallel reaction monitoring-based targeted proteomics

ABSTRACT: Untargeted proteomics based precise diagnosis and typing of amyloid deposits are crucial for guiding patient management. However, it may not be suitable for early-stage renal amyloidosis with minor damages. Here, we aimed to develop parallel reaction monitoring (PRM)-based targeted proteomics to achieve high sensitivity and specificity in identifying early-stage renal immunoglobulin-derived amyloidosis.This study demonstrates that the utility of these prioritized peptides in PRM-based targeted proteomics ensure highly sensitive and reliable for identifying early-stage renal amyloidosis. Owing to the development and clinical application of this method, rapid acceleration of the early diagnosis, and typing of renal amyloidosis is expected.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney

SUBMITTER: Yuan Li  

LAB HEAD: Guolan Xing

PROVIDER: PXD038265 | Pride | 2023-03-10

REPOSITORIES: Pride

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Precise diagnosis and typing of early-stage renal immunoglobulin-derived amyloidosis by label-free quantification of parallel reaction monitoring-based targeted proteomics.

Li Yuan Y   Zhang Ying Y   Zhou Xinjin X   Xue Xinli X   Wang Muxi M   Kang Dedong D   Zhou Yali Y   Hu Ruimin R   Quan Songxia S   Xing Guolan G   Yang Jinghua J  

BMC nephrology 20230310 1

<h4>Background</h4>Early diagnosis and typing are crucial for improving the prognosis of patients with renal amyloidosis. Currently, Untargeted proteomics based precise diagnosis and typing of amyloid deposits are crucial for guiding patient management. Although untargeted proteomics achieve ultra-high-throughput by selecting the most abundant eluting cationic peptide precursors in series for tandem MS events, it lacks in sensitivity and reproducibility, which may not be suitable for early-stage  ...[more]

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