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Proteomic analysis of SARS-CoV-2 particles unveils a key role of G3BP proteins in viral assembly

ABSTRACT: The aim of this project was to identify cellular factors exclusively present in SARS-CoV-2 virion preparations.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Virginie Salnot

LAB HEAD: Sarah Gallois-Montbrun

PROVIDER: PXD038321 | Pride | 2024-01-22

REPOSITORIES: Pride

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Proteomic analysis of SARS-CoV-2 particles unveils a key role of G3BP proteins in viral assembly.

Murigneux Emilie E Softic Laurent L Aubé Corentin C Grandi Carmen C Judith Delphine D Bruce Johanna J Le Gall Morgane M Guillonneau François F Schmitt Alain A Parissi Vincent V Berlioz-Torrent Clarisse C Meertens Laurent L Hansen Maike M K MMK Gallois-Montbrun Sarah S

Nature communications 20240120 1

Considerable progress has been made in understanding the molecular host-virus battlefield during SARS-CoV-2 infection. Nevertheless, the assembly and egress of newly formed virions are less understood. To identify host proteins involved in viral morphogenesis, we characterize the proteome of SARS-CoV-2 virions produced from A549-ACE2 and Calu-3 cells, isolated via ultracentrifugation on sucrose cushion or by ACE-2 affinity capture. Bioinformatic analysis unveils 92 SARS-CoV-2 virion-associated h ...[more]

PMID: 38245532

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