Dataset Information

The central mediator of auxin-triggered phosphorylation in plants is a RAF-like kinase.

ABSTRACT: The plant signaling molecule auxin triggers both fast and slow cellular responses across the plant lineage, including both land plants and algae. We discovered an ultra-fast proteome-wide phosphorylation response to auxin across 5 land plant and algal species, converging on a core group of shared target proteins. We find conserved rapid physiological responses to auxin in the same species and identified a RAF-like protein kinase as a central mediator of auxin-triggered phosphorylation across species.

INSTRUMENT(S): Q Exactive HF-X, Orbitrap Exploris 480

ORGANISM(S): Penium Margaritaceum Physcomitrella Patens Subsp. Patens (moss) Marchantia Polymorpha Arabidopsis Thaliana (mouse-ear Cress) Klebsormidium Nitens

TISSUE(S): Root, Thallus, Cell Culture

SUBMITTER: Sjef Boeren

LAB HEAD: Dolf Weijers

PROVIDER: PXD038363 | Pride | 2024-01-26

REPOSITORIES: Pride

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Publications

RAF-like protein kinases mediate a deeply conserved, rapid auxin response.

Kuhn Andre A Roosjen Mark M Mutte Sumanth S Dubey Shiv Mani SM Carrillo Carrasco Vanessa Polet VP Boeren Sjef S Monzer Aline A Koehorst Jasper J Kohchi Takayuki T Nishihama Ryuichi R Fendrych Matyáš M Sprakel Joris J Friml Jiří J Weijers Dolf D

Cell 20231220 1

The plant-signaling molecule auxin triggers fast and slow cellular responses across land plants and algae. The nuclear auxin pathway mediates gene expression and controls growth and development in land plants, but this pathway is absent from algal sister groups. Several components of rapid responses have been identified in Arabidopsis, but it is unknown if these are part of a conserved mechanism. We recently identified a fast, proteome-wide phosphorylation response to auxin. Here, we show that t ...[more]

PMID: 38128538

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Project description:During the evolution of life on Earth, the conquest of land by plants played a pivotal role producing a boost in land biomass, a substantial drop in atmospheric CO2, an increase in oxygen and the emergence of new terrestrial habitats facilitating land colonization by animals. Therefore, the characterization of the molecular mechanisms that allowed plant terrestralization is a cornerstone in evolutionary studies. Viridiplantae or the green lineage is divided into two clades Chlorophyta or green microalgae and Streptophyta that in turn splits into Embryophyta or land plants and Charophyta. The latest are mainly considered aquatic algae although some facultative terrestrial species has been identified. Charophyta are generally accepted as the extant algal species most closely related to current land plants and, therefore, they are used in evolutionary studies on plant terrestralization. High light irradiance was one of the major stressors that ancestral charophytic algae needed to overcome during the transition from aquatic to terrestrial environments. In this study, we have chosen the facultative terrestrial early charophytic alga Klebsormidium nitens to perform an integrative transcriptomic and metabolomic analysis under high light in order to unveil key mechanisms involved in the early steps of plants terrestralization. We found a fast chloroplast retrograde signaling possibly mediated by reactive oxygen species and the inositol polyphosphate 1-phosphatase (SAL1) and 3′-phosphoadenosine-5′-phosphate (PAP) pathways inducing gene expression and accumulation of specific metabolites. Systems used by both Chlorophyta and Embryophyta were activated such as the xanthophyll cycle with an accumulation of zeaxanthin and protein folding and repair mechanisms constituted by NADPH-dependent thioredoxin reductases, thioredoxin-disulfide reductases and peroxiredoxins. Similarly, cyclic electron flow, specifically the pathway dependent on Proton Gradient Regulation 5, was strongly activated under high light. We detected a simultaneous co-activation of the non-photochemical quenching mechanisms based on LHC-like Stress Related protein and the photosystem II subunit S that are specific to Chlorophyta and Embryophyta respectively. Exclusive Embryophyta systems for the synthesis, sensing and response to the phytohormone auxin were also activated under high light in Klebsormidium leading to an increase in auxin content with the concomitant accumulation of amino acids such as tryptophan, histidine and phenylalanine.

Dataset Information

The central mediator of auxin-triggered phosphorylation in plants is a RAF-like kinase.

Publications

RAF-like protein kinases mediate a deeply conserved, rapid auxin response.

Similar Datasets

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