Proteomics

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Global Neuropeptidome Profiling in Response to Post-traumatic Stress Disorder in Rat

ABSTRACT: Post-traumatic stress disorder (PTSD) is a psychiatric disorder that affects the health of a wide range of the population. However, its underlying neurophysiological mechanisms remain unclear due to the limited understanding of neuronal signaling molecules such as neuropeptides. Here, we developed mass spectrometry (MS) based qualitative and quantitative analysis to profile the neuropeptides in the control and predator odor exposure rat PTSD model group. In total, 628 unique neuropeptides can be identified across 5 fear circuitry-related brain regions. Meanwhile, brain region-specific changes of serval neuropeptide families including granin, ProSAAS, opioid peptide, cholecystokinin, and tachykinin can be observed in the PTSD group. Neuropeptides from the same protein precursor were also found to vary in different brain regions. This study reveals for the first time the interaction between neuropeptides and severe stress, providing insights into the molecular mechanism of PTSD and corresponding therapeutic strategy.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Min Ma  

LAB HEAD: lingjun Li

PROVIDER: PXD038470 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
BLA_Ctrl_BR1.raw Raw
BLA_Ctrl_BR2.raw Raw
BLA_Ctrl_BR3.raw Raw
BLA_PTSD_BR1.raw Raw
BLA_PTSD_BR2.raw Raw
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Publications

Global Neuropeptidome Profiling in Response to Predator Stress in Rat: Implications for Post-Traumatic Stress Disorder.

Wu Wenxin W   Ma Min M   Ibarra Angel Erbey AE   Lu Gaoyuan G   Bakshi Vaishali P VP   Li Lingjun L  

Journal of the American Society for Mass Spectrometry 20230705 8

Traumatic stress triggers or exacerbates multiple psychiatric illnesses, including post-traumatic stress disorder (PTSD). Nevertheless, the neurophysiological mechanisms underlying stress-induced pathology remain unclear, in part due to the limited understanding of neuronal signaling molecules, such as neuropeptides, in this process. Here, we developed mass spectrometry (MS)-based qualitative and quantitative analytical strategies to profile neuropeptides in rats exposed to predator odor (an eth  ...[more]

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