FAIMS-DIA Proteomics of LIRIL2R-Deficient Induced Regulatory T Cells
Ontology highlight
ABSTRACT:
INSTRUMENT(S):
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Primary Cell, T Cell, Blood
SUBMITTER: 
Kedar Batkulwar
LAB HEAD: Riitta Lahesmaa
PROVIDER: PXD038477 | Pride | 2024-05-16
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| 20221026_LncTreg1_LNA1_R1.htrms | Other | |||
| 20221026_LncTreg1_LNA1_R1.raw | Raw | |||
| 20221026_LncTreg1_LNA1_R2.htrms | Other | |||
| 20221026_LncTreg1_LNA1_R2.raw | Raw | |||
| 20221026_LncTreg1_LNA1_R3.htrms | Other |
Items per page: 5 1 - 5 of 17 |
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Project description:The CD4+ regulatory T (Treg) cell lineage comprises thymus-derived (t)Treg cells and peripherally induced (p)Treg cells. As a model for Treg cells, studies employ TGF-β-induced (i)Treg cells generated from CD4+ conventional T (Tconv) cells in vitro. Here, we describe the relationship of iTreg cells to tTreg and Tconv cells. Proteomic analysis revealed that iTreg, tTreg and Tconv cell populations each have a unique protein expression pattern. iTreg cells had very limited overlap in protein expression with tTreg cells, regardless of cell activation status and instead shared signaling and metabolic proteins with Tconv cells. tTreg cells had a uniquely modest response to CD3/CD28-mediated stimulation. As a benchmark, we used a previously defined proteomic signature that sets ex vivo naïve and effector phenotype Treg cells apart from Tconv cells and includes unique Treg cell properties (Cuadrado et al., Immunity, 2018). This Treg cell core signature was largely absent in iTreg cells. We also used a proteomic signature that distinguishes ex vivo effector Treg cells from Tconv cells and naïve Treg cells. This effector Treg cell signature was partially present in iTreg cells. In conclusion, iTreg cells are distinct from tTreg cells and share limited features with ex vivo Treg cells at the proteomic level.
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