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Proteome-wide mapping of early events connected to oxidative stress in Arabidopsis

ABSTRACT: Changes in environmental conditions are associated with the fast accumulation of reactive oxygen species, which may rapidly generate an oxidative environment. For efficient survival, plants respond by rapid changes in protein turnover, gene expression and posttranslational protein modifications. For this reason, the regulation of ROS levels requires rapid protective mechanisms including of antioxidant defense. Here, we aimed to delineate the early events associated with the response of Arabidopsis to methyl viologen on proteome level.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Whole Body

SUBMITTER: Tibor Pechan

LAB HEAD: Tibor Pechan

PROVIDER: PXD038495 | Pride | 2024-01-26

REPOSITORIES: Pride

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Methyl viologen-induced changes in the Arabidopsis proteome implicate PATELLIN 4 in oxidative stress responses.

Melicher Pavol P Dvořák Petr P Řehák Jan J Šamajová Olga O Pechan Tibor T Šamaj Jozef J Takáč Tomáš T

Journal of experimental botany 20240101 1

The photosynthesis-induced accumulation of reactive oxygen species in chloroplasts can lead to oxidative stress, triggering changes in protein synthesis, degradation, and the assembly/disassembly of protein complexes. Using shot-gun proteomics, we identified methyl viologen-induced changes in protein abundance in wild-type Arabidopsis and oxidative stress-hypersensitive fsd1-1 and fsd1-2 knockout mutants, which are deficient in IRON SUPEROXIDE DISMUTASE 1 (FSD1). The levels of proteins that are ...[more]

PMID: 37728561

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Arabidopsis thaliana

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| PRJNA108021 | ENA

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