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Proteome characterization of HCMV virus microenvironment

ABSTRACT: The control of host processes which influence cell-cell communication is central to determining the outcome of a virus infection in tissue. Despite this, it remains unclear how cells either in close or distant proximity to an infected cell differentially respond to the primary infection. Here, we established a virus infection microenvironment to resolve molecular features and functional consequences of cell spatial address within this localized niche by mass spectrometry.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Human Betaherpesvirus 5

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Bokai Song

LAB HEAD: Ileana Cristea

PROVIDER: PXD038547 | Pride | 2023-05-11

REPOSITORIES: Pride

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Intercellular communication within the virus microenvironment affects the susceptibility of cells to secondary viral infections.

Song Bokai B Sheng Xinlei X Justice Joshua L JL Lum Krystal K KK Metzger Peter J PJ Cook Katelyn C KC Kostas James C JC Cristea Ileana M IM

Science advances 20230510 19

Communication between infected cells and cells in the surrounding tissue is a determinant of viral spread. However, it remains unclear how cells in close or distant proximity to an infected cell respond to primary or secondary infections. We establish a cell-based system to characterize a virus microenvironment, distinguishing infected, neighboring, and distal cells. Cell sorting, microscopy, proteomics, and cell cycle assays allow resolving cellular features and functional consequences of proxi ...[more]

PMID: 37163594

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