Mechanical tension-induced spatial control of angiogenesis during lung regeneration

ABSTRACT: Mechanical forces altered after unilateral pneumonectomy (PNX) control post-PNX lung growth. Here we demonstrate that post-PNX vascular formation is stimulated at the peripheral region of the mouse lung requiring focal adhesion protein, paxillin, while inhibition of mechanical tension attenuates the effects. Expression of paxillin increases in endothelial cells (ECs) and alveolar epithelial type 2 (AT2) cells after PNX, which upregulates angiogenic factor, angiopoietin-2 (Ang2) in ECs and Ang1 in AT2 cells to dictate vascular formation at the peripheral region. Proliferation of general capillary ECs increases at the peripheral region of the post-PNX mouse lungs. Extracellular matrix proteins that impact cell mechanical responses are spatially differentially expressed in the post-PNX mouse lungs, which drives paxillin expression, leading to vascular formation at the peripheral region of mouse lungs. These results suggest that cooperative action of mechanosensitive signaling in ECs and AT2 cells mediates spatial control of vascular formation during regenerative lung growth.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Lung

DISEASE(S): Disease Free

SUBMITTER: Priscilla Kyi  

LAB HEAD: Akiko Mammoto

PROVIDER: PXD038600 | Pride | 2026-10-19

REPOSITORIES: Pride